Ibicuruzwa

Ibisobanuro birambuye:

Ikizamini cya Innovita® 2019-nCoV IgM / IgG kigamije kumenya igice cya kabiri cyo gutahura antibodiyite zanduza igitabo cyitwa coronavirus (2019-nCoV) muri serumu yumuntu, plasma cyangwa amaraso yose.
2019-nCoV ikubiyemo poroteyine enye zingenzi zubaka: S proteine, E proteyine, proteine ​​M na proteine ​​N.Agace ka RBD ka poroteyine irashobora guhuza na selile yumuntu yakira ACE2.Kutabogama antibody bivuga ubushobozi bwo guhuza na virusi, hanyuma ugahagarika virusi itera umubiri gutera indwara.Kumenya antibody itabogamye irashobora gukoreshwa mugusuzuma ibimenyetso byanduye virusi.

Ihame:

Igikoresho ni amarushanwa ya zahabu ya immunochromatografiya kugirango amenye antibodiyite zangiza muri 2019-nCoV muri serumu yumuntu, plasma cyangwa amaraso yose.Nyuma yikigereranyo gishyizwe kumurongo neza, niba antibodiyite zitabogamye zihari murugero, antibodies zidafite aho zibogamiye zizakorana na zahabu ya colloidal yanditseho RBD antigen kugirango ikore urwego rwumubiri, kandi urubuga rutabogamye rwa antigen RBD rwarafunzwe.Noneho ubudahangarwa bw'umubiri hamwe na antigen ya RBD bitabujije guhuza antibody byimuka kuri nitrocellulose.Iyo bageze mukarere kipimisha (T umurongo), antigen ya RBD itiriwe ihambira antibodiyite izakorana na antigen ya ACE2 yometse kuri nitrocellulose hanyuma ikore umurongo wumutuku.Iyo intumbero yo kutabuza antibodies irenze igipimo cyo hasi cyo gutahura, umurongo wumutuku-umutuku uba woroshye kurenza umurongo ugenzura (C umurongo) cyangwa ntamurongo wumutuku wumutuku wakozwe, ibisubizo nibyiza.Iyo kwibumbira hamwe kwa antibodiyite biri munsi yurwego rwo hasi rwo gutahura cyangwa nta antibodiyite zidafite aho zibogamiye, umurongo wumutuku-umutuku wijimye kuruta umurongo ugenzura, ibisubizo ni bibi.
Tutitaye ku kumenya niba urugero rurimo antibodiyite za 2019-nCoV zidafite aho zibogamiye, mugihe inkoko ya colloidal yanditseho inkoko IgY antibody yimukiye kumurongo ugenzura (C umurongo), izafatwa nihene irwanya inkoko antibody antibody yabanjirije umurongo ugenzura (C umurongo), umurongo wijimye-umutuku urakorwa.Igenzura ry'umurongo (C umurongo) rikoreshwa nkigenzura ryimikorere.Imirongo igenzura igomba guhora igaragara mubisubizo Windows niba inzira yikizamini ikozwe neza kandi reagent ikora nkuko byateganijwe ..

Ibigize:

Uburyo bwo Kwipimisha:

1. Fungura umufuka wa aluminium hanyuma ukuremo cassette yikizamini.
2. Koresha 40μL ya serumu / plasma cyangwa 60μL yuzuye yamaraso neza.
3. Koresha 40μL (ibitonyanga 2) urugero rwiza kurugero rwiza.
4. Shyira mubushyuhe bwicyumba (15 ℃ ~ 30 ℃) muminota 15-20, hanyuma usome ibisubizo.

Ibisubizo Ibisobanuro:

1. Ibyiza: Iyo ibara ryumurongo wa T ryoroheje kurenza umurongo wa C cyangwa mugihe nta murongo wa T, byerekana ibyiza byo kutabuza antibodi.
2. Ibibi: Iyo ibara ryumurongo wa T ryijimye cyangwa rihwanye numurongo wa C, byerekana nabi kubuza antibodi.
3. Ntibyemewe: Iyo umurongo wa C unaniwe kugaragara, uko umurongo wa T ugaragara cyangwa utagaragara, ikizamini nticyemewe.Subiramo ikizamini hamwe nikizamini gishya.