Ibicuruzwa

Ibisobanuro birambuye:

Innovita® 2019-nCoV IgM / IgG Ikizamini kigamije gusuzuma no gukurikirana epidemiologi yindwara ziterwa na roman coronavirus (2019-nCoV).ORF1ab na N gene yo muri 2019-nCoV igaragazwa neza bivuye mu muhogo no mu maraso ya alveolar lavage yakusanyirijwe mu bakekwaho kuba barwaye umusonga, abarwayi bafite ibibazo, abandi bakeneye kwisuzumisha.
Ibisubizo by'ibizamini by'iki gikoresho ni ibyavuzwe gusa.Birasabwa gukora isesengura ryuzuye ryimiterere hashingiwe ku miterere y’umurwayi n’ibindi bizamini bya laboratoire.

Ihame:

Iki gikoresho gikoresha intambwe imwe ihinduranya transcription polymerase urunigi (RT-PCR) tekinoroji yo gutahura intego yibintu bya coronavirus (2019-nCoV) ORF1ab gene, N gene, hamwe nabantu bakurikirana imbere.Primers yihariye na taqman probe zakozwe mukarere kabitswe.

Ibigize:

Icyitonderwa: 1. Ibice bitandukanye bya reagent ntibigomba kuvangwa.
2. Igenzura ryiza nubugenzuzi bwiza ntibigomba gukurwaho

Uburyo bwo Kwipimisha:

1. Gukuramo aside nucleique:
Ibikoresho byo gukuramo ibicuruzwa bya RNA birahari, gukuramo amasaro ya magneti no gukuramo inkingi birasabwa kuri iki gikoresho.
2. Gutegura reaction ivanze:

Kuramo reaction ya 2019-nCoV Kuvanga A / B hanyuma ugumane ubushyuhe bwicyumba kugeza bidakonje;
Fata ibice bihuye (Reaction Mix A 16.5μL / T, Ivangavanga B 3.5μL / T) hanyuma uvange, hanyuma ugabanye buri reaction ya PCR hamwe na 20μL / tube;
Ongeraho 5μL yicyitegererezo cya RNA cyangwa kugenzura nabi cyangwa kugenzura neza, hanyuma upfundikire igituba;
Shyira umuyoboro wa reaction mu gikoresho cya fluorescence PCR, hanyuma ushireho ibibi / byiza kugenzura hamwe nicyitegererezo cyibisubizo bya RT-PCR ukurikije amabwiriza yo gukoresha ibikoresho.
Andika icyitegererezo cyo gutondekanya

3.RT-PCR protocole:

Igenamiterere risabwa: